Media Formulations

Media preparation recipes are available upon request. Please contact [email protected] for assistance.

Name

 

DMEM High Glucose

DMEM Low Glucose

DMEM Low Glucose

Minimum Essential Medium

RPMI 1640

RPMI 1640

F12 Ham's Nutrient Mixture

Alpha Minimum Essential Medium

Alpha Minimum Essential Medium

DMEM High Glucose/Ham's F-12 50% mix

DMEM Low Glucose/Ham's F-12 50% mix

MEM PAK

Iscove’s Modified Dulbecco’s Medium

M-199, EBSS

McCoy’s 5A Media

McCoy’s 5A Media

PBS, CMF, 10X

Trypsin-EDTA, 0.05%, in Saline A (STV)

Eagle's MEM

 

Description

 

DME H-21, 4.5 g/L glucose

DME H-16, 1 g/L glucose

DME H-16, 3 g/L glucose

MEM Eagles with EBS

without Hepes

with 25 mM Hepes

F12 Ham's Nutrient Mixture

with nucleosides

without nucleosides

DME H-21/F-12, without Hepes

DME H-16/F-12, without Hepes

modified MEM EBSS w/o L-Glutamine

(IMDM)

with Earle’s Balanced Salts Solution (EBSS)

Modified without Tricine

Modified with Tricine

10X Stock (D-PBS, Ca++ & Mg++ Free Media)

 

with Earle's BSS (Balanced Salt Solution), without L-Glutamine

Catalog number

 

CCFAA005

CCFAA001

CCFAA004

CCFAC001

CCFAE001

CCFAE002

CCFAB002

CCFAC007

CCFAC006

CCFAA010

CCFAA009

CCFAC009

CCFDY002

CCFAX001

CCFAD001

CCFAD002

CCFAL004

CCFGP002

CCFAC002

 

Buffers

Dulbecco's PBS

Dulbecco's PBS

Hank's Balanced Salt Solution

Hank's Balanced Salt Solution

 

1X D-PBS, with Ca++ and Mg++

D-PBS, Ca++ and MG++ free

1X HBSS

1X HBSS without Ca++ and Mg++

CCFAL001

CCFAL003

CCFAJ001

CCFAJ003

 

Enzymes

Trypsin/EDTA

Trypsin/EDTA

0.05% trypsin

0.25% trypsin

CCFGP001

CCFGP003

 

Additives to stock media

Fungizone (Amphotericin B), 5 ml

Fungizone (Amphotericin B), 50 ml

Antibiotic-Antimycotic Solution, 50 ml

Sodium Pyruvate, 11 mg/ml, 25 ml

L-Glutamine, 200 mM, 25 ml

Penicillin-Streptomycin Solution, 5 ml

Penicillin-Streptomycin Solution, 50 ml

Penicillin-Streptomycin Solution, 100 ml

MEM Non-Essential Amino Acids

Cell Preservation Media, 10 ml

Cell Preservation Media, 50 ml

H.T. (Hypoxanthine and Thymidine)

H.A.T. (Hypoxanthine, Aminopterin and Thymidine)

Insulin, 2 ml

1 M HEPES, 25 ml

1 M HEPES, 100 ml

Gentamicin Solution, 50 mg/ml, 10 ml

100X solution

100X solution

100X Penicillin / Streptomycin / Fungizone

100X solution

100X solution

100X solution

100X solution

100X solution

100X solution

MEM CPM

MEM CPM

100X solution

100X solution

1 mg/ml

 

 

100X solution

CCFGH001

CCFGH002

CCFGG001

CCFGE001

CCFGB002

CCFGK001

CCFGK003

CCFGK004

CCFGA001

CCFHH001

CCFHH002

CCFGC002

CCFGC001

CCFHK001

CCFGL001

CCFGL002

CCFGI001