Media Formulations

Click on any catalog number to view components of each recipe  

Name

 

DMEM High Glucose

DMEM Low Glucose

DMEM Low Glucose

Minimum Essential Medium

RPMI 1640

RPMI 1640

F12 Ham's Nutrient Mixture

Alpha Minimum Essential Medium

Alpha Minimum Essential Medium

DMEM High Glucose/Ham's F-12 50% mix

DMEM Low Glucose/Ham's F-12 50% mix

MEM PAK

Iscove’s Modified Dulbecco’s Medium

M-199, EBSS

McCoy’s 5A Media

McCoy’s 5A Media

Description

 

DME H-21, 4.5 g/L glucose

DME H-16, 1 g/L glucose

DME H-16, 3 g/L glucose

MEM Eagles with EBS

without Hepes

with 25 mM Hepes

F12 Ham's Nutrient Mixture

with nucleosides

without nucleosides

DME H-21/F-12, without Hepes

DME H-16/F-12, without Hepes

modified MEM EBSS w/o L-Glutamine

(IMDM)

with Earle’s Balanced Salts Solution (EBSS)

Modified without Tricine

Modified with Tricine

 

Buffers

Dulbeccos PBS

Dulbeccos PBS

Hank's Balanced Salt Solution

Hank's Balanced Salt Solution

1X D-PBS, with Ca++ and Mg++

D-PBS, Ca++ and MG++ free

1X HBSS

1X HBSS without Ca++ and Mg++

 

Enzymes

Trypsin/EDTA

Trypsin/EDTA

0.05% trypsin

0.25% trypsin

 

Additives to stock media

Fungizone (Amphotericin B), 5 ml

Fungizone (Amphotericin B), 50 ml

Antibiotic-Antimycotic Solution, 50 ml

Sodium Pyruvate, 11 mg/ml, 25 ml

L-Glutamine, 200 mM, 25 ml

Penicillin-Streptomycin Solution, 5 ml

Penicillin-Streptomycin Solution, 50 ml

Penicillin-Streptomycin Solution, 100 ml

MEM Non-Essential Amino Acids

Cell Preservation Media, 10 ml

Cell Preservation Media, 50 ml

H.T. (Hypoxanthine and Thymidine)

H.A.T. (Hypoxanthine, Aminopterin and Thymidine)

Insulin, 2 ml

1 M HEPES, 25 ml

1 M HEPES, 100 ml

Gentamicin Solution, 50 mg/ml, 10 ml

100X solution

100X solution

100X Pencillin / Streptomycin / Fungizone

100X solution

100X solution

100X solution

100X solution

100X solution

100X solution

MEM CPM

MEM CPM

100X solution

100X solution

1 mg/ml

 

 

100X solution